About Us
Biomedical Research Center
The Sohail University has taken a leap forward to enrich its educational resources, by setting up Biomedical Research Center (BRC). The Center is temperature controlled research facility being established at 7th floor in Medicare Hospital. It is, divided into cell culture, molecular biology, biochemistry and cell biology sections. The Center is equipped with state of the art laboratory equipment and has following features:
Biomedical Research Center
- Gene expression analysis, genotyping and rare allele detection
- Biosafety level-2 unit supports working with cell lines, primary human cells culture and organisms/ biological samples of moderate risk.
- Imaging facility for immunohistochemistry and immunofluorescence
- Protein separation and detection facility
- Nucleic acid separation and detection facility
- Sophisticated equipment: Refrigerated centrifuges and CO2 Incubator
- Basic equipment: centrifuges, water baths, hotplate, pH meters and vortexes
- Computers with full access to the internet
- Epoxy resin laboratory work surfaces, chemical and flame resistant secured cabinets
- Storage facility with ULT freezers and liquid nitrogen tanks for biological samples.
- Stored items include consumables, chemicals and reagents, and biological samples (Human samples, imported cell lines, plasmid vectors and antibodies).
- Safe environment (Restricted access, logging and monitoring and surveillance round the clock).
- Conference room equipped with audio visual (AV) technology.
Location
Medicare Cardiac And General Hospital,
The Sohail University, Medicare Campus
Shaheed-e-Millat Road Karachi, Pakistan
Contact
Prof. Dr Rizwana S.Waraich
Dean Faculty of Basic & Applied Sciences
Founding Director Biomedical Research Center
Laboratory Research Services
Nucleic Acid Extraction
Nucleic Acid Amplification
Nucleic Acid Electrophores
Nucleic Acid Detection
Protein Extraction
Protein Electrophores
Protein Detection
Genomic Instability Assays
Microscopy
Bio-assays
Cytogenetic
Ultra-Law temperature Storage Facilities
Spectroscopy
Cell Culture
Rate List
Research Experimental Services
| S.No. | Services offered | No. Of Samples | Cost |
| 1 | DNA extraction | 1 Sample | 2700 |
| 2 | RNA extraction | 1 Sample | 6400 |
| 3 | RFLP | 1 Sample | 5000 |
| 4 | cDNA synthesis | 1 Sample | 4000 |
| 5 | PCR | 1 target | 1800 |
| 6 | RT-PCR | 1 target | 2000 |
| 7 | Primer designing and ordering | 1 primer set | 1000 |
| 8 | Agarose gel electrophoresiss | 18 Samples | 4500 |
| 9 | Nucleic acid analysis by nanodrop | 16 Samples | 1200 |
| 10 | Protein Extraction | 1 Sample | 2000 |
| 11 | Protein quantification by Bradford assay | 20 Samples | 2000 |
| 12 | SDS-PAGE | 8 Samples | 4500 |
| 13 | Western blotting | 8 Samples | 20000 |
| 14 | Tissue processing and sectioning for histology | 1 Sample | 2300 |
| 15 | Immunohistochemistry | 1 Sample | 5000 |
| 16 | Immunofluorescence | 1 Sample | 6000 |
| 17 | Immunocytochemistry | 1 Sample | 3000 |
| 18 | Fluorescence Microscopy and imaging | 1 Sample | 4000 |
| 19 | DAPI staining | 1 Sample | 5000 |
| 20 | Dihydroethidium Staining | 1 Sample | 5000 |
| 21 | DIC, Bright field and Phase Contrast Microscopy | 1 Sample | 1700 |
| 22 | Periodic acid & Schiff staining of tissue / cells and imaging | 1 Sample | 5000 |
| 23 | Hematoxylin & Eosin (H&E) staining | 1 Sample | 4000 |
| 24 | Cells and Tissues Culture | (6 well plate) | 3500 |
| 25 | MTT assay (cell viability assay) | (6 well plate) | 3500 |
| 26 | Cells Lines-Passaging and Growth | 5000 | |
| 27 | ELISA from serum and tissue samples | Depends upon sample type |
Research Equipment Services
| S.No. | Services offered | No. Of Samples | Cost |
| 1 | Ultra-low freezer (-80°C) storage | 1 Box (5”x5”) | 1000/month |
| 2 | -196 Liquid nitrogen storage | 5 cryovials | 2000/month |
| 3 | Freezer (-20°C) storage | 1 Box (5”x5”) | 1000/month |
| 4 | Refrigerator 5°C storage | 1 Box (5”x5”) | 800/month |
| 5 | Deionize and Distilled (DD) water | 1litre | 300/Litre |
| 6 | Conventional Thermal cycler | per use | 3000 |
| 7 | Real time Thermal cycler | 90 samples | 5000 |
| 8 | Multimode microplate (ELISA Reader) | per use | 2000 |
| 9 | Nanodrop | per use | 1500 |
| 10 | Gel documentation | per use | 1500 |
| 11 | Agarose electrophoresis assembly | per use | 1200 |
| 12 | SDS-PAGE electrophoresis assembly | per use | 1200 |
| 13 | Electronic pipettor (pipette aid) | per use | 100 |
| 14 | Analytical balance | per use | 300 |
| 15 | Refrigerated centrifuge (for microtubes & falcon tubes) | per use | 500 |
| 16 | Homogenizer | per use | 300 |
| 17 | Microtome | per use | 400 |
| 18 | Embedding system | per use | 400 |
| 19 | Slide warmer | per use | 300 |
| 20 | Inverted microscope (bright field/phase contrast) | per use | 1500 |
| 21 | Fluorescence microscope | per use | 3000 |
| 22 | CO2 incubator | per use | 1000 |
| 23 | Biosafety cabinet II (cell culture) | per use | 1000 |
| 24 | Western Blot scanner | per use | 1000 |
Molecular Diagnostics Services
Corona Virus RNA Qualitative PCR
Corona Virus RNA Qualitative PCR
Pre-Flight COVID-19 PCR Testing
Pre-Flight COVID-19 PCR Testing
Hepatitis C Virus RNA Genotyping
Hepatitis C Virus RNA Genotyping
Hepatitis C Virus RNA Qualitative PCR
Hepatitis C Virus RNA Qualitative PCR
Hepatitis C Virus RNA Quantitative PCR (Viral Load)
Hepatitis C Virus RNA Quantitative PCR (Viral Load)
Hepatitis B Virus DNA Qualitative PCR
Hepatitis B Virus DNA Qualitative PCR
Hepatitis B Virus DNA Quantitative PCR (Viral Load)
Hepatitis B Virus DNA Quantitative PCR (Viral Load)
Hepatitis B Virus DNA Genotyping
Hepatitis B Virus DNA Genotyping
Dengue Virus RNA Qualitative PCR
Dengue Virus RNA Qualitative PCR
Dengue Virus RNA Genotyping PCR Dengue Virus RNA Genotyping PCR
Dengue Virus RNA Genotyping PCR Dengue Virus RNA Genotyping PCR
Swine Influenza Virus (H1N1) RNA Qualitative PCR
Swine Influenza Virus (H1N1) RNA Qualitative PCR
Mycobacterium Tuberculosis Qualitative PCR
Mycobacterium Tuberculosis Qualitative PCR
Mycobacterium Tuberculosis Qualitative PCR
Mycobacterium Tuberculosis Qualitative PCR
Alpha-Thalassemia
Alpha-Thalassemia
Xmn Polymorphism
Xmn Polymorphism
Profile Thalassemia Genetic Profile
Profile Thalassemia Genetic Profile
Fetal (CVS) Genetic Screening
Fetal (CVS) Genetic Screening
Hb Electrophoresis
Hb Electrophoresis
Beta Thalassemia
Beta Thalassemia
Rate List
| S.No. | Name Of PCR Test | Price | Delivery Time |
| 1 | Corona virus RNA qualitative PCR | 6500 | 24 hours |
| 2 | Hepatitis B virus DNA qualitative PCR | 4400 | After 3 days |
| 3 | Hepatitis B virus DNA qualitative PCR(Viral Load) | 4400 | After one week |
| 4 | Hepatitis B virus DNA genotyping | 9200 | After 5 days |
| 5 | Hepatitis C virus DNA qualitative PCR | 4200 | After 3 days |
| 6 | Hepatitis C virus RNA quantitative PCR (Viral Load) | 4200 | After one week |
| 7 | Hepatitis C virus RNA genotyping | 9300 | After 5 days |
| 8 | Dengue virus RNA qualitative PCR | 3500 | 24 hour |
| 9 | Dengue virus RNA genotyping PCR | 5300 | After 3 days |
| 10 | Mycobactrium tuberculosis qualitative PCR | 4000 | After 3 days |
| 11 | Swine Influenza Virus (H 1 N 1) RNA qualitative PCR | 8000 | After 3 days |
| 12 | Alpha Thalassemia | 5900 | After 15 days |
| 13 | Beta Thalassemia | 6900 | After 15 days |
| 14 | Xmn Polumorphism | 7900 | After 15 days |
| 15 | Fetal (CVS) Genetic Screening | 11900 | After 15 days |
| 16 | Thalassemia Genetic Profile | 15900 | After 15 days |
| 17 | Hb Electrophoresis | 2000 | After 4 days |
Collaborations
Gallery
Our Location
Map View
Contact Info
- Biomedical Research Center, 7th Floor, Sohail University
- 021-34931886 Ext: 841
- brc@sohailuniversity.edu.pk