The Sohail University has taken a leap forward to enrich its educational resources, by setting up Biomedical Research Center (BRC). The Center is temperature controlled research facility being established at 7th floor in Medicare Hospital. It is, divided into cell culture, molecular biology, biochemistry and cell biology sections. The Center is equipped with state of the art laboratory equipment and has following features:
Medicare Cardiac And General Hospital,
The Sohail University, Medicare Campus
Shaheed-e-Millat Road Karachi, Pakistan
Prof. Dr Rizwana S.Waraich
Dean Faculty of Basic & Applied Sciences
Founding Director Biomedical Research Center
S.No. | Services offered | No. Of Samples | Cost |
1 | DNA extraction | 1 Sample | 2700 |
2 | RNA extraction | 1 Sample | 6400 |
3 | RFLP | 1 Sample | 5000 |
4 | cDNA synthesis | 1 Sample | 4000 |
5 | PCR | 1 target | 1800 |
6 | RT-PCR | 1 target | 2000 |
7 | Primer designing and ordering | 1 primer set | 1000 |
8 | Agarose gel electrophoresiss | 18 Samples | 4500 |
9 | Nucleic acid analysis by nanodrop | 16 Samples | 1200 |
10 | Protein Extraction | 1 Sample | 2000 |
11 | Protein quantification by Bradford assay | 20 Samples | 2000 |
12 | SDS-PAGE | 8 Samples | 4500 |
13 | Western blotting | 8 Samples | 20000 |
14 | Tissue processing and sectioning for histology | 1 Sample | 2300 |
15 | Immunohistochemistry | 1 Sample | 5000 |
16 | Immunofluorescence | 1 Sample | 6000 |
17 | Immunocytochemistry | 1 Sample | 3000 |
18 | Fluorescence Microscopy and imaging | 1 Sample | 4000 |
19 | DAPI staining | 1 Sample | 5000 |
20 | Dihydroethidium Staining | 1 Sample | 5000 |
21 | DIC, Bright field and Phase Contrast Microscopy | 1 Sample | 1700 |
22 | Periodic acid & Schiff staining of tissue / cells and imaging | 1 Sample | 5000 |
23 | Hematoxylin & Eosin (H&E) staining | 1 Sample | 4000 |
24 | Cells and Tissues Culture | (6 well plate) | 3500 |
25 | MTT assay (cell viability assay) | (6 well plate) | 3500 |
26 | Cells Lines-Passaging and Growth | 5000 | |
27 | ELISA from serum and tissue samples | Depends upon sample type |
S.No. | Services offered | No. Of Samples | Cost |
1 | Ultra-low freezer (-80°C) storage | 1 Box (5”x5”) | 1000/month |
2 | -196 Liquid nitrogen storage | 5 cryovials | 2000/month |
3 | Freezer (-20°C) storage | 1 Box (5”x5”) | 1000/month |
4 | Refrigerator 5°C storage | 1 Box (5”x5”) | 800/month |
5 | Deionize and Distilled (DD) water | 1litre | 300/Litre |
6 | Conventional Thermal cycler | per use | 3000 |
7 | Real time Thermal cycler | 90 samples | 5000 |
8 | Multimode microplate (ELISA Reader) | per use | 2000 |
9 | Nanodrop | per use | 1500 |
10 | Gel documentation | per use | 1500 |
11 | Agarose electrophoresis assembly | per use | 1200 |
12 | SDS-PAGE electrophoresis assembly | per use | 1200 |
13 | Electronic pipettor (pipette aid) | per use | 100 |
14 | Analytical balance | per use | 300 |
15 | Refrigerated centrifuge (for microtubes & falcon tubes) | per use | 500 |
16 | Homogenizer | per use | 300 |
17 | Microtome | per use | 400 |
18 | Embedding system | per use | 400 |
19 | Slide warmer | per use | 300 |
20 | Inverted microscope (bright field/phase contrast) | per use | 1500 |
21 | Fluorescence microscope | per use | 3000 |
22 | CO2 incubator | per use | 1000 |
23 | Biosafety cabinet II (cell culture) | per use | 1000 |
24 | Western Blot scanner | per use | 1000 |
S.No. | Name Of PCR Test | Price | Delivery Time |
1 | Corona virus RNA qualitative PCR | 6500 | 24 hours |
2 | Hepatitis B virus DNA qualitative PCR | 4400 | After 3 days |
3 | Hepatitis B virus DNA qualitative PCR(Viral Load) | 4400 | After one week |
4 | Hepatitis B virus DNA genotyping | 9200 | After 5 days |
5 | Hepatitis C virus DNA qualitative PCR | 4200 | After 3 days |
6 | Hepatitis C virus RNA quantitative PCR (Viral Load) | 4200 | After one week |
7 | Hepatitis C virus RNA genotyping | 9300 | After 5 days |
8 | Dengue virus RNA qualitative PCR | 3500 | 24 hour |
9 | Dengue virus RNA genotyping PCR | 5300 | After 3 days |
10 | Mycobactrium tuberculosis qualitative PCR | 4000 | After 3 days |
11 | Swine Influenza Virus (H 1 N 1) RNA qualitative PCR | 8000 | After 3 days |
12 | Alpha Thalassemia | 5900 | After 15 days |
13 | Beta Thalassemia | 6900 | After 15 days |
14 | Xmn Polumorphism | 7900 | After 15 days |
15 | Fetal (CVS) Genetic Screening | 11900 | After 15 days |
16 | Thalassemia Genetic Profile | 15900 | After 15 days |
17 | Hb Electrophoresis | 2000 | After 4 days |