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About Us

Biomedical Research Center

The Sohail University has taken a leap forward to enrich its educational resources, by setting up Biomedical Research Center (BRC). The Center is temperature controlled research facility being established at 7th floor in Medicare Hospital. It is, divided into cell culture, molecular biology, biochemistry and cell biology sections. The Center is equipped with state of the art laboratory equipment and has following features:

Biomedical Research Center

  • Gene expression analysis, genotyping and rare allele detection
  • Biosafety level-2 unit supports working with cell lines, primary human cells culture and organisms/ biological samples of moderate risk.
  • Imaging facility for immunohistochemistry and immunofluorescence
  • Protein separation and detection facility
  • Nucleic acid separation and detection facility
  • Sophisticated equipment: Refrigerated centrifuges and CO2 Incubator
  • Basic equipment: centrifuges, water baths, hotplate, pH meters and vortexes
  • Computers with full access to the internet
  • Epoxy resin laboratory work surfaces, chemical and flame resistant secured cabinets
  • Storage facility with ULT freezers and liquid nitrogen tanks for biological samples.
  • Stored items include consumables, chemicals and reagents, and biological samples (Human samples, imported cell lines, plasmid vectors and antibodies).
  • Safe environment (Restricted access, logging and monitoring and surveillance round the clock).
  • Conference room equipped with audio visual (AV) technology.

Location

Medicare Cardiac And General Hospital,
The Sohail University, Medicare Campus
Shaheed-e-Millat Road Karachi, Pakistan​

Contact

Prof. Dr Rizwana S.Waraich
Dean Faculty of Basic & Applied Sciences
Founding Director Biomedical Research Center

Laboratory Research Services

Nucleic Acid Extraction

Nucleic Acid Amplification

Nucleic Acid Electrophores

Nucleic Acid Detection

Protein Extraction

Protein Electrophores

Protein Detection

Genomic Instability Assays

Microscopy

Bio-assays

Cytogenetic

Ultra-Law temperature Storage Facilities

Spectroscopy

Cell Culture

Rate List

Research Experimental Services

S.No. Services offered No. Of Samples Cost
1 DNA extraction 1 Sample 2700
2 RNA extraction 1 Sample 6400
3 RFLP 1 Sample 5000
4 cDNA synthesis 1 Sample 4000
5 PCR 1 target 1800
6 RT-PCR 1 target 2000
7 Primer designing and ordering 1 primer set 1000
8 Agarose gel electrophoresiss 18 Samples 4500
9 Nucleic acid analysis by nanodrop 16 Samples 1200
10 Protein Extraction 1 Sample 2000
11 Protein quantification by Bradford assay 20 Samples 2000
12 SDS-PAGE 8 Samples 4500
13 Western blotting 8 Samples 20000
14 Tissue processing and sectioning for histology 1 Sample 2300
15 Immunohistochemistry 1 Sample 5000
16 Immunofluorescence 1 Sample 6000
17 Immunocytochemistry 1 Sample 3000
18 Fluorescence Microscopy and imaging 1 Sample 4000
19 DAPI staining 1 Sample 5000
20 Dihydroethidium Staining 1 Sample 5000
21 DIC, Bright field and Phase Contrast Microscopy 1 Sample 1700
22 Periodic acid & Schiff staining of tissue / cells and imaging 1 Sample 5000
23 Hematoxylin & Eosin (H&E) staining 1 Sample 4000
24 Cells and Tissues Culture (6 well plate) 3500
25 MTT assay (cell viability assay) (6 well plate) 3500
26 Cells Lines-Passaging and Growth 5000
27 ELISA from serum and tissue samples Depends upon sample type

Research Equipment Services

S.No. Services offered No. Of Samples Cost
1 Ultra-low freezer (-80°C) storage 1 Box (5”x5”) 1000/month
2 -196 Liquid nitrogen storage 5 cryovials 2000/month
3 Freezer (-20°C) storage 1 Box (5”x5”) 1000/month
4 Refrigerator 5°C storage 1 Box (5”x5”) 800/month
5 Deionize and Distilled (DD) water 1litre 300/Litre
6 Conventional Thermal cycler per use 3000
7 Real time Thermal cycler 90 samples 5000
8 Multimode microplate (ELISA Reader) per use 2000
9 Nanodrop per use 1500
10 Gel documentation per use 1500
11 Agarose electrophoresis assembly per use 1200
12 SDS-PAGE electrophoresis assembly per use 1200
13 Electronic pipettor (pipette aid) per use 100
14 Analytical balance per use 300
15 Refrigerated centrifuge (for microtubes & falcon tubes) per use 500
16 Homogenizer per use 300
17 Microtome per use 400
18 Embedding system per use 400
19 Slide warmer per use 300
20 Inverted microscope (bright field/phase contrast) per use 1500
21 Fluorescence microscope per use 3000
22 CO2 incubator per use 1000
23 Biosafety cabinet II (cell culture) per use 1000
24 Western Blot scanner per use 1000

Molecular Diagnostics Services

Corona Virus RNA Qualitative PCR

Corona Virus RNA Qualitative PCR

Pre-Flight COVID-19 PCR Testing

Pre-Flight COVID-19 PCR Testing

Hepatitis C Virus RNA Genotyping

Hepatitis C Virus RNA Genotyping

Hepatitis C Virus RNA Qualitative PCR

Hepatitis C Virus RNA Qualitative PCR

Hepatitis C Virus RNA Quantitative PCR (Viral Load)

Hepatitis C Virus RNA Quantitative PCR (Viral Load)

Hepatitis B Virus DNA Qualitative PCR

Hepatitis B Virus DNA Qualitative PCR

Hepatitis B Virus DNA Quantitative PCR (Viral Load)

Hepatitis B Virus DNA Quantitative PCR (Viral Load)

Hepatitis B Virus DNA Genotyping

Hepatitis B Virus DNA Genotyping

Dengue Virus RNA Qualitative PCR

Dengue Virus RNA Qualitative PCR

Dengue Virus RNA Genotyping PCR Dengue Virus RNA Genotyping PCR

Dengue Virus RNA Genotyping PCR Dengue Virus RNA Genotyping PCR

Swine Influenza Virus (H1N1) RNA Qualitative PCR

Swine Influenza Virus (H1N1) RNA Qualitative PCR

Mycobacterium Tuberculosis Qualitative PCR

Mycobacterium Tuberculosis Qualitative PCR

Mycobacterium Tuberculosis Qualitative PCR

Mycobacterium Tuberculosis Qualitative PCR

Alpha-Thalassemia

Alpha-Thalassemia

Xmn Polymorphism

Xmn Polymorphism

Profile Thalassemia Genetic Profile

Profile Thalassemia Genetic Profile

Fetal (CVS) Genetic Screening

Fetal (CVS) Genetic Screening

Hb Electrophoresis

Hb Electrophoresis

Beta Thalassemia

Beta Thalassemia

Rate List

S.No. Name Of PCR Test Price Delivery Time
1 Corona virus RNA qualitative PCR 6500 24 hours
2 Hepatitis B virus DNA qualitative PCR 4400 After 3 days
3 Hepatitis B virus DNA qualitative PCR(Viral Load) 4400 After one week
4 Hepatitis B virus DNA genotyping 9200 After 5 days
5 Hepatitis C virus DNA qualitative PCR 4200 After 3 days
6 Hepatitis C virus RNA quantitative PCR (Viral Load) 4200 After one week
7 Hepatitis C virus RNA genotyping 9300 After 5 days
8 Dengue virus RNA qualitative PCR 3500 24 hour
9 Dengue virus RNA genotyping PCR 5300 After 3 days
10 Mycobactrium tuberculosis qualitative PCR 4000 After 3 days
11 Swine Influenza Virus (H 1 N 1) RNA qualitative PCR 8000 After 3 days
12 Alpha Thalassemia 5900 After 15 days
13 Beta Thalassemia 6900 After 15 days
14 Xmn Polumorphism 7900 After 15 days
15 Fetal (CVS) Genetic Screening 11900 After 15 days
16 Thalassemia Genetic Profile 15900 After 15 days
17 Hb Electrophoresis 2000 After 4 days

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