About Us
Biomedical Research Center
The Sohail University has taken a leap forward to enrich its educational resources, by setting up Biomedical Research Center (BRC). The Center is temperature controlled research facility being established at 7th floor in Medicare Hospital. It is, divided into cell culture, molecular biology, biochemistry and cell biology sections. The Center is equipped with state of the art laboratory equipment and has following features:
Biomedical Research Center
- Gene expression analysis, genotyping and rare allele detection
- Biosafety level-2 unit supports working with cell lines, primary human cells culture and organisms/ biological samples of moderate risk.
- Imaging facility for immunohistochemistry and immunofluorescence
- Protein separation and detection facility
- Nucleic acid separation and detection facility
- Sophisticated equipment: Refrigerated centrifuges and CO2 Incubator
- Basic equipment: centrifuges, water baths, hotplate, pH meters and vortexes
- Computers with full access to the internet
- Epoxy resin laboratory work surfaces, chemical and flame resistant secured cabinets
- Storage facility with ULT freezers and liquid nitrogen tanks for biological samples.
- Stored items include consumables, chemicals and reagents, and biological samples (Human samples, imported cell lines, plasmid vectors and antibodies).
- Safe environment (Restricted access, logging and monitoring and surveillance round the clock).
- Conference room equipped with audio visual (AV) technology.
Location
Medicare Cardiac And General Hospital,
The Sohail University, Medicare Campus
Shaheed-e-Millat Road Karachi, Pakistan
Contact
Prof. Dr Rizwana S.Waraich
Dean Faculty of Basic & Applied Sciences
Founding Director Biomedical Research Center
Laboratory Research Services
Nucleic Acid Extraction
Nucleic Acid Amplification
Nucleic Acid Electrophores
Nucleic Acid Detection
Protein Extraction
Protein Electrophores
Spectroscopy
Protein Detection
Microscopy
Bio-assays
Ultra-Law temperature Storage Facilities
Cell Culture
Genomic Instability Assays
Cytogenetic
Commercial Research Services - 2025
Molecular Biology Services
| S.No. | Services offered |
| 1 | DNA extraction (Manual method) |
| 2 | DNA extraction (Kit method) |
| 3 | RNA extraction (Manual method) |
| 4 | RNA extraction from cells (Manual method) |
| 5 | RNA extraction (Kit method) |
| 6 | Nucleic acid concentration and purity determination by nanodrop |
| 7 | Gene amplification (Conventional PCR) |
| 8 | Gene expression (RT-PCR) |
| 9 | cDNA synthesis |
| 10 | Protein isolation |
| 11 | Protein isolation from cells |
| 12 | Agarose gel electrophoresis of DNA |
| 13 | Protein electrophoresis (SDS-PAGE) |
| 14 | Protein concentration determination by Bradford assay |
| 15 | Coomassie staining of protein gel |
| 16 | Western blotting |
Immunoassays Services
| S.No. | Services offered |
| 1 | ELISA |
Histology & Microscopy Services
| S.No. | Services offered |
| 1 | Animal handling and tissue harvesting |
| 2 | Tissue processing |
| 3 | H & E staining |
| 4 | Periodic acid & Schiff staining |
| 5 | DHE/DAPI staining of tissue slide / cells in multiwell plate |
| 6 | Immunohistochemistry (IHC) |
| 7 | Immunofluorescence (IF) |
| 8 | Immunocytochemistry (ICC) |
Cell Culture Services
| S.No. | Services offered |
| 1 | Basic culturing of cells |
| 2 | Basic passaging & seeding of cells |
| 3 | MTT assay |
| 4 | Primary fibroblast culturing |
| 5 | PBMC isolation and culture with MGO stimulation |
| 6 | Trophoblast isolation and culture |
| 7 | Chondrocytes isolation and culture |
| 8 | Mesenchymal stem cell culture |
Other Services
| S.No. | Services offered |
| 1 | Refrigerator 4°C storage |
| 2 | Ultra-low freezer (-80°C) storage |
| 3 | Freezer (-20°C) storage |
| 4 | Ultra-low freezer (-40°C) storage-month |
| 5 | Deionize Water |
Equipment Services
| S.No. | Services offered |
| 1 | Ultra-low freezer (-80°C) storage |
| 2 | Freezer (-40°C) storage |
| 3 | Deionize and Distilled (DD) water |
| 4 | Conventional Thermal cycler |
| 5 | Real time Thermal cycler |
| 6 | Multimode microplate (ELISA Reader) |
| 7 | Nanodrop |
| 8 | Gel documentation |
| 9 | Agarose electrophoresis assembly |
| 10 | SDS-PAGE electrophoresis assembly |
| 11 | Western Blot scanner |
| 12 | Embedding system |
| 13 | Microtome |
| 14 | Fluorescence microscope |
Molecular Diagnostics Services
Corona Virus RNA Qualitative PCR
Section: Molecular pathology
Sample requirement: Nasal swab
Methodology: Real time PCR
Reporting time: after 12 hours
Sample requirement: Nasal swab
Methodology: Real time PCR
Reporting time: after 12 hours
HLA-B27
Qualitative PCR
Section: Molecular pathology
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 5 days
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 5 days
HSV-1&2 DNA Qualitative PCR
Section: Molecular pathology
Sample requirement: CSF/ Plasma
Methodology: Real time PCR
Reporting time: after 2 days
Sample requirement: CSF/ Plasma
Methodology: Real time PCR
Reporting time: after 2 days
Hepatitis C Virus RNA Qualitative PCR
Section: Molecular pathology
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 3 days
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 3 days
Hepatitis C Virus RNA Quantitative PCR (Viral Load)
Section: Molecular pathology
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 1 week
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 1 week
Hepatitis B Virus DNA Qualitative PCR
Section: Molecular pathology
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 3 days
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 3 days
Hepatitis B Virus DNA Quantitative PCR (Viral Load)
Section: Molecular pathology
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 1 week
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 1 week
Congo Virus Qualitative PCR
Section: Molecular pathology
Sample requirement: Nasal swab
Methodology: Real time PCR
Reporting time: after 12 hours
Sample requirement: Nasal swab
Methodology: Real time PCR
Reporting time: after 12 hours
Dengue Virus RNA Qualitative PCR
Section: Molecular pathology
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 24 hours
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 24 hours
Influenza Virus (H1N1) RNA Qualitative PCR
Section: Molecular pathology
Sample requirement: Nasal swab
Methodology: Real time PCR
Reporting time: after 3 days
Sample requirement: Nasal swab
Methodology: Real time PCR
Reporting time: after 3 days
Mycobacterium Tuberculosis Qualitative PCR
Section: Molecular pathology
Sample requirement: Nasal swab
Methodology: Real time PCR
Reporting time: after 3 days
Sample requirement: Nasal swab
Methodology: Real time PCR
Reporting time: after 3 days
Chikungunya Virus RNA qualitative PCR
Section: Molecular pathology
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 24 hours
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 24 hours
Malarial parasite DNA Qualitative PCR
Section: Molecular pathology
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 24 hours
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 24 hours
Factor V DNA Qualitative PCR
Section: Molecular pathology
Sample requirement: Plasma
Methodology: Real time PCR
Reporting time: after 5 days
Sample requirement: Plasma
Methodology: Real time PCR
Reporting time: after 5 days
MRSA DNA Qualitative PCR
Section: Molecular pathology
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 5 days
Sample requirement: 5cc Blood in EDTA tube
Methodology: Real time PCR
Reporting time: after 5 days
Profile Thalassemia Genetic Profile
Profile Thalassemia Genetic Profile
Fetal (CVS) Genetic Screening
Fetal (CVS) Genetic Screening
Hb Electrophoresis
Hb Electrophoresis
Beta Thalassemia
Beta Thalassemia
Rate List
| S.No. | Name Of PCR Test | Price | Delivery Time |
| 1 | Corona virus RNA qualitative PCR | 6500 | 24 hours |
| 2 | Hepatitis B virus DNA qualitative PCR | 4400 | After 3 days |
| 3 | Hepatitis B virus DNA qualitative PCR(Viral Load) | 4400 | After one week |
| 4 | Hepatitis B virus DNA genotyping | 9200 | After 5 days |
| 5 | Hepatitis C virus DNA qualitative PCR | 4200 | After 3 days |
| 6 | Hepatitis C virus RNA quantitative PCR (Viral Load) | 4200 | After one week |
| 7 | Hepatitis C virus RNA genotyping | 9300 | After 5 days |
| 8 | Dengue virus RNA qualitative PCR | 3500 | 24 hour |
| 9 | Dengue virus RNA genotyping PCR | 5300 | After 3 days |
| 10 | Mycobactrium tuberculosis qualitative PCR | 4000 | After 3 days |
| 11 | Swine Influenza Virus (H 1 N 1) RNA qualitative PCR | 8000 | After 3 days |
| 12 | Alpha Thalassemia | 5900 | After 15 days |
| 13 | Beta Thalassemia | 6900 | After 15 days |
| 14 | Xmn Polumorphism | 7900 | After 15 days |
| 15 | Fetal (CVS) Genetic Screening | 11900 | After 15 days |
| 16 | Thalassemia Genetic Profile | 15900 | After 15 days |
| 17 | Hb Electrophoresis | 2000 | After 4 days |
Collaborations
Gallery
Contact Details
Call:
- 021-111-001-786, 021-34935008-9
- Ext:1700
- rizwanas.waraich@sohailuniversity.edu.pk
- Aisha.kamal@sohailuniversity.edu.pk
- Gulnaz.khan@sohailuniversity.edu.pk
- brc@sohailuniversity.edu.pk
Address
- 22-23 Shaheed-e-Millat Road, 7th floor Medicare Cardiac and General Hospital, Karachi-74000 Pakistan